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Radial migration assay was performed as previously described [30,31]. In a blinded fashion, various U87Gal-1 clones were analyzed and compared to U87GFP controls and parental U87MG cells. Of each clone, 2500 cells were allowed to sediment through a cooled manifold onto laminin-coated cell culture wells (Creative Scientific Methods, Inc., Phoenix, AZ). The manifold and slide were incubated togethe
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T least one of these symptoms was said to be bothersome,Hemminki et al. BMC Women's Health 2012, 12:44 http://www.biomedcentral.com/1472-6874/12/Page 3 ofthe woman was categorized as having bothersome symptoms of that group.Table 2 Proportions ( ) of women with vasomotor symptoms and bothersome symptoms1 in past two weeks, at each survey (cross-sectional analysis)Recruitment Women
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Few of our U87 galectin1 clones. Parental U87MG cells, along with galectin-1 and acGFP-only clones were injected into the right caudate/putamen complex of nude mice. Tumors overexpressing galectin-1 shortened survival of their hosts compared to their parental counterparts (Figure 5). A few animals (7/20) bearing tumors expressing acGFP alone eventually exhibited neurological symptoms. The examina
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